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Fig. 7 | Cancer Cell International

Fig. 7

From: Decoding the prognostic significance of integrator complex subunit 9 (INTS9) in glioma: links to TP53 mutations, E2F signaling, and inflammatory microenvironments

Fig. 7

The functional validation was achieved by downregulating INTS9 expression using siINTS9. (A) The presence of INTS9 was ascertained in various tumor cell lines, with increased expression compared to normal brain lysates. (B-C) After siINTS9 administration, INTS9 expression decreased at protein and transcriptomic levels. (D) Decreased INTS9 was identified after being supplemented with siINTS9, and the arrowhead indicated tumor cells. (E) Investigated cancer hallmark-related and proliferation markers; notably, GBM8401 cells showed reduced mtTFA and N-cadherin but increased LC3BII and PCNA, highlighted with asterisk symbols. (F-J) Cell cycle analysis revealed a significant decrease in the S phase and G2/M in GBM8401 cells, with a slight increase in the sub-G1 phase. (K-L) Compared to the control group, an increased apoptosis proportion in the GBM8401 cell line when supplemented with siINTS9. (M-O) However, no noticeable change in cytosolic ROS was observed in the tested cell lines. Bars, mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001

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Cancer Cell International

ISSN: 1475-2867

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