Fig. 9

The INTS9 expression in the single-cell sequencing and 12 cell-states analyses (A-C) INTS9 expression strongly correlated with tumor (arrow) and myeloid lineages (arrowhead) in IDH wildtype and (D-F) mutant astrocytoma. The T cell also expressed high INTS9 in the IDH wildtype but equivocal in the IDH mutant group (asterisk). (C) Significant differences were found among tumor-to-myeloid, tumor-to-T cells, and tumor-to-reactive glia. (F) In IDH mutant astrocytoma, significant differences were found between tumor-to-myeloid and tumor-to-reactive glia. (G) INTS9 positively correlated with tumor-stem and tumor-proliferative-stem cells across all subtypes. A slight difference among subtypes is the contrasting correlation between INTS9 and differentiated tumor cells in IDH mutant and wildtype groups. INTS9 displayed a negative relationship with specific stromal cells across all three subtypes but a positive correlation with fibroblasts only in IDH wildtype and oligodendroglioma. For the immune cells, INTS9 positively correlated with myeloid cells, while a significant association with T cell quantities appeared only in IDH wildtype astrocytoma