Fig. 9
Validation of the expression of the 2-gene signature. A T-SNE describes the expression profiles of CKS2 and LYZ in the single cells obtained from myeloma tissues. Every point on the graph represents a single cell. The plot can be downloaded from the CancerSEA database. B BCKS2 and LYZ mRNA expression in newly diagnosed, non-treated multiple myeloma patients were measured by microarray from GSE39754. C The relative expression levels of the 2 genes compared with GAPDH in normal (n = 14) and MM (n = 14) samples (*, p < 0.05; **, p < 0.01). D Knockdown of CKS2 reduced the cell viability of MM1.S myeloma cell line. E Knockdown of CKS2 increased the cell apoptosis of MM1.S myeloma cell line. F Invasion and migration ratio of MM cell toward two groups through Transwell membranes (5-mm pore size) were assessed. Independent experiments were performed 3 times. n = 5 per group (**, p < 0.01; ****, p < 0.0001; vs. CONTROL; ns, no significance)