Fig. 3
SEMA3C is associated with stem cell-like properties and promotes self-renewal and tumorigenicity in PDAC cells. (A) Left panels: Expression patterns of stem cell markers, such as CD44 and CD24, were compared in BxPC-3 cells transfected control siRNA, SEMA3C siRNA1, and SEMA3C siRNA2 by flow cytometry. Right panel: The percentage of CD44high24high cells. (B) Left panels: Expression patterns of CD44 and CD24 in MIA PaCa-2 control and SEMA3C-overexpressing cells using flow cytometry. Right panel: The percentage of CD44high24high cells. The sphere formation rate in BxPC-3 cells treated with control siRNA, SEMA3C siRNA1, and SEMA3C siRNA2 (C), and in MIA PaCa-2 cells control and SEMA3C-overexpressing cells (D). Colony formation assay with BxPC-3 cells treated with control siRNA, SEMA3C siRNA1, and SEMA3C siRNA2 (E), and MIA PaCa-2 control and SEMA3C-overexpression cells (F)