Fig. 5

Preclinical trial to confirm GnP efficacy using the orthotopic transplantation mouse model. (A) The experimental design of the randomized trial (untreated group / GnP group; gemcitabine (GEM, 120 mg/kg) and nab-paclitaxel (nab-PTX, 120 mg/kg)) in the orthotopic transplantation model injected with control- and SEMA3C shRNA-transduced PKCY cells. (B) Paraffin-embedded orthotopic tumor tissues were sectioned and stained with H&E. (C) The indicated excised tumor of the orthotopic model. (D) Pancreatic tumor volumes are represented as box-plot histograms. Each volume represents the mean ± SEM. (E) Waterfall plot indicating changes in the volume compared with the baseline (mean tumor volume of each no treated group). (F) Image of a mouse with malignant ascites just before euthanasia. (G) The frequency of malignant ascites in mice just before sacrifice