Fig. 1
Isolation of XN from the H. lupulus crude extract. A P-TLC chromatography image shows a separated fraction containing XN fraction (F), baseline (BC), and a solvent fraction (Sf). B Verification of XN identity obtained from the H. lupulus fraction using the FTIR method. The graph shows the FT-IR curve for the isolated XN. Accordingly, O-H, C-H, C = O, C-O, C = C, C-O-C, and the aromatic ring in XN functional groups are the expected wave numbers in this diagram. C Verification of extracted XN identity through the H-NMR method. According to the radio frequencies, XN–specific peaks emerged following the application of the H-NMR method in which criteria were as follows: H-NMR (300 HZ): H: 1.61 (3 H, s), 1.70 (3 H, s), 3.14 (2 H, d, J = 5.37), 3.87 (3 H, s), 5.14 (1 H, t, J = 5.37), 6.09 (1 H, s), 6.85 (2 H, d, J = 5.01), 7.58 (2 H, d, J = 5.01), 7.68 (1 H, AB4, J = 9.31), 7.77 (1 H, AB4, J = 9.31), 10.09 (1 H, s), 10.59 (1 H, s), 14.66 (1 H, s)