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Fig. 2 | Cancer Cell International

Fig. 2

From: Xanthohumol hinders invasion and cell cycle progression in cancer cells through targeting MMP2, MMP9, FAK and P53 genes in three-dimensional breast and lung cancer cells culture

Fig. 2

Spheroid formation, inhibitory effects of XN on viability and invasion assay on MCF-7 and A549. A, B Shows the images of MCF-7 and A549 spheroids produced using the HD method. Images are taken by inverted microscope at 0, 1, and 2 days (Original magnification 20×) and the scale bar is 50 μm. C Shows the SEM photographs of produced spheroids (Original magnification 1000×) and the scale bar is 50 μm. D Graphs show the normal size distribution for the produced spheroids in both cell lines. Spheroids were in the range of 98.60 ± 4.25 and 94.17 ± 3.89, for the MCF-7 and A549 cells, respectively. E, F, G, H There is a significant difference in the cell viability under 2D and 3D conditions at various times (24, 48, and 72 h) and concentrations (from 9.37 µM to 300 µM).The significant differences comparing the control (i.e., untreated) cells are represented by star(s) as follows: *p < .05, **p < .01, ***p < .001, ****p < .0001). K, L Microfilaments are decreased in XN-treated MCF-7 K, and L A549 spheroid

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Cancer Cell International

ISSN: 1475-2867

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