Fig. 3

XN-treatment effect on the cell cycle progression, death cell and cancer-related gene expression of MCF-7 and A549 cells. A, B Flow cytometry results show that the Sub-G1 has been increased, while the G1 cell population has been reduced in the MCF-7 and A549 cells following the treatment. C, D Representative histogram of cell cycle analysis based on flow cytometry. E , F Shows Propidium iodide (PI)-Annexin V-FITC binding assay using MCF-7 and A549 cells. Results indicated a significant elevation of necrosis and Late-apoptosis versus G XN-untreated-MCF-7 cells and H XN-untreated-A549 cells. I RT-qPCR results showed that BAX, P21, and P53 genes have been significantly upregulated while the BCL2 gene has been downregulated following the XN treatment in MCF-7 cells. Also, MMP9, MMP2, NFKB2, FAK, MDR1, and SURVIVIN genes were significantly downregulated at this condition and P21, P53 genes increased versus control. J RT-qPCR results showed that BAX, and P53 genes have been upregulated while other genes BCL2 gene have been downregulated following the XN-treatment in A549 cells. P53 genes increased versus control. [All the tests were compared to the untreated control 48 h after treatment with 12.37 µM of XN for MCF-7 and 31.17 µM of XN for A549 and statistical significances are expressed as p < .05 (*); p < .01 (**); p < .001 (***); p < .0001 (****)]