Xanthohumol hinders invasion and cell cycle progression in cancer cells through targeting MMP2, MMP9, FAK and P53 genes in three-dimensional breast and lung cancer cells culture

Table 1 Sequences of the primers used in qRT-PCR

Gene name	Product length(bp)	Primer sequence
P53	181	F: GGAGTATTTGGATGACAGAA R: GATTACCACTGGAGTCTT
P21	150	F: CCAGCATGACAGATTTCTA R: AGACACACAAACTGAGACTAAG
BAX	187	F: CAAACTGGTGCTCAAG R: CACAAAGATGGTCACGGT
MDR1	161	F: TGGACAAGCACTGAAAGATAA R: TTCCTCAAAGAGTTTCTGTATG
NFkB1	182	F: GTGCTGGAGTTCAGGATAAC R:GTGGATGATTGCTAAGTGTAAGA
NFkB2	223	R: GAGTTGCTACAACCCAGGT F: ACAGTGGGATAGGTCTTTC
SURVIVIN	144	F: GACCACCGCATCTCTACAT R: CTCCTTGAAGCAGAAGAAAC
FAK	228	F: ATAGAACTTGGACGATGTATT R: TGACGCATTGTTAAGGCTT
MMP2	136	F: GCTCGTGCCTTCCAAG R: AGTCCGTCCTTACCGTC
MMP9	116	F: CGGACCAAGGATACAGTTT R: CTCAGTGAAGCGGTACATA
ABCG2	166	F: AACTCAGTTTATCCGTGGT R: CTGCTTAGACATCCT TTTCA
BCL2	70	F: GATAACGGAGGCTGGGA R: CAGGAGAAATCAAACAGAGG
β-ACTIN	215	F: CTTCCTTCCTGGGCA R: GTCTTTGCGGATGTCCA

ABCG2 ATP-binding cassette subfamily G member 2, BAX Bcl-2-associated X protein, BCL2 B-cell leukemia/lymphoma 2 protein, FAK Focal adhesion kinase, MDR1 ATP Binding Cassette Subfamily B Member 1, MMP2 Matrix metalloproteinase 2, MMP9 Matrix metalloproteinase 9, NFκB1 Nuclear Factor Kappa B Subunit 1, NFκB2 Nuclear Factor Kappa B Subunit 2, P2 Cyclin Dependent Kinase Inhibitor 1 A,
P53 Tumor Protein P53

ISSN: 1475-2867