Fig. 5

Downregulation of TCIRG1 expression inhibits the proliferation, migration and invasion of ccRCC cell lines. (A-B) RT-qPCR results of TCIRG1 (A) and representative Western blot images with relative gray values and histograms (B) for TCIRG1 mRNA and protein in different ccRCC cell lines (769-P, 786-O, OS-RC-2, A498, ACHN), using normal human renal cell line (HK-2) as normal controls. (C-D) RT-qPCR results of TCIRG1 (C) and representative Western blot images with relative gray values and histograms (D) showed that siRNA silenced the mRNA and protein expression of TCIRG1 in OS-RC-2 or 769P, respectively. (E) CCK-8 was used to detect the proliferation of OS-RC-2 or 769P cells during TCIRG1 knockdown. The proliferation rate showed a multiple change relative to the control group. (F) In the renal cell carcinoma cell lines (OS-RC-2 and 769-P) transfected with TCIRG1 (control and si-TCIRG1), the migration ability was detected by Transwell, and the invasion ability was detected by matrigel. (All p values are defined as: *p < 0.05, **p < 0.01 and ***p < 0.001). ccRCC, clear cell renal cell carcinoma; RT-qPCR, real-time polymerase chain reaction; siRNA, Small interfering RNA; CCK‐8, Cell Counting Kit‐8; FITC, fluorescein isothiocyanate; PI, propidium iodide