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Fig. 6 | Cancer Cell International

Fig. 6

From: Glycolysis-related biomarker TCIRG1 participates in regulation of renal cell carcinoma progression and tumor immune microenvironment by affecting aerobic glycolysis and AKT/mTOR signaling pathway

Fig. 6

Downregulation of TCIRG1 expression inhibits migration, invasion, glycolysis processes, and the AKT/mTOR signaling pathway in the ccRCC cell lines. (A) Annexin V-FITC/PI double-staining of OS-RC-2 or 769-P cells with or without TCIRG1 knockdown was performed, and flow cytometry assays were employed to detect the percentage of apoptotic cells. (B-E) Glucose consumption (B), lactate production (C), pyruvate production (D), and intracellular ATP production (E) were detected in TCIRG1 transfected (control and si-TCIRG1) renal cell carcinoma cell lines (OS-RC-2 and 769-P). (F) The expression level of AKT/mTOR signaling pathway related proteins in renal cell carcinoma cell lines (OS-RC-2 and 769-P) transfected with TCIRG1 (control and si-TCIRG1) was detected. (All p values are defined as: *p < 0.05, **p < 0.01 and ***p < 0.001). ccRCC, clear cell renal cell carcinoma; mTOR, Mechanistic Target of Rapamycin; ATP, Association of Tennis Professionals

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Cancer Cell International

ISSN: 1475-2867

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