Fig. 3

ADAM8 is expressed in all breast cancer subtypes. Freshly cut consecutive TMA slides containing breast cancer patient primary tumor samples were subjected to IHC with ADP2 vs isotype-matched control IgG2b at 1:50 dilutions. H&E staining was used to confirm tissue quality and exclude depleted samples. A Representative images of ADP2-stained TNBC samples with increasing ADAM8 expression and associated H-scores are shown. Staining with IgG2b was negative as expected (not shown). Upper panels: whole core images, scale bar: 200 μm; lower panels: magnified images of same samples, scale bar: 50 μm. B Representative images of ADP2 IHC staining for ADAM8 in breast cancer patient samples of non-TNBC HR−/HER2+, HR+/HER2− and HR+/HER2+ subtypes. Specimens with high H-scores (e.g., 250–300) are shown. Upper panels: whole core images, scale bar: 200 μm; lower panels: magnified images of same samples, scale bar: 50 μm