Fig. 1
Hsa_circRNA_102051 was overexpressed in metastatic CRC samples. (A) GSE147597 was downloaded from the GEO database and processed difference analysis. 20 CRC samples from patients with or without liver metastasis were collected and detected. (B) Sequencing results showing the back-splice junction sequences of circ_102051. (C) Expression levels of candidate circRNAs in selected chips. Hsa_circRNA_102051 revealed the most significant overexpression in tumor samples compared to the normal ones. (D) RNase R identification of hsa_circRNA_102051. (E) RNA expression levels of hsa_circRNA_102051 and TADA2A along with time. TADA2A expression displayed a gradual drop while hsa_circRNA_102051 kept in high level. (F) TADA2A expression in colon and rectum adenocarcinoma tissues. TADA2A was overexpressed in colorectal tumors compared to normal tissues. (G) Expression of hsa_circRNA_102051 in CRC cell lines was higher than that in FHC cell line. (H) Expression of hsa_circRNA_102051 in tissues of Grade III-IV was higher than that in Grade 1-II. (I) QRT-PCR was used to detect the expression of has_circRNA_102051 in the nucleus and cytoplasm. (J) IHC staining of tumor and adjacent samples with corresponding hsa_circRNA_102051 expression. Hsa_circRNA_102051 was overexpressed in highly heterogeneous tumor tissues. (K) FISH testing of hsa_circRNA_102051 in FHC and SW480 cells. hsa_circRNA_102051 expression was mainly in cytoplasm and enhanced in SW480 cells. Compared to normal tissues or cells, *P < 0.05