Fig. 2

Hsa_circRNA_102051 promoted the proliferation, migration and invasion of CRC cells. (A) Transfection efficiency in SW480 and HT-29 cells. Expression of hsa_circRNA_102051 was inhibited with si-hsa_circRNA_102051 transfection in SW480 while enhanced with hsa_circRNA_102051 transfection in HT-29, compared to the si-NC or NC group. (B) CCK-8 assays in SW480 and HT-29 cells. (C) EdU cell proliferation testing in SW480 and HT-29 cells. Cell proliferation was inhibited in CRC cells when hsa_circRNA_102051 was downregulated while activated with additional hsa_circRNA_102051. (D) TUNEL fluorescence assay in SW480 and HT-29 cells. SW480 cells transfected with si-hsa_circRNA_102051 displayed a higher apoptosis rate, while apoptosis of HT-29 cells transfected with hsa_circRNA_102051 showed a decrease but the difference was not statistically significant. (E) Cell invasion in CRC cell lines was inhibited by si-hsa_circRNA_102051 while enhanced by hsa_circRNA_102051 transfection. (F) Expression of E-cadherin, N-cadherin and vimentin in SW480 and HT-29 cells. Invasion and migration of CRC cells were blocked by si-hsa_circRNA_102051 while enhanced by external hsa_circRNA_102051. Compared to si-NC or NC group, ^*P < 0.05