Fig. 3

Hsa_circRNA_102051 was capable to promote tumor growth and metastasis in vivo. (A) Hsa_circRNA_102051 was successfully knocked down by sh-circ transfection in nude mice. (B) Tumor volume during 25 days and final weight in xenograft mice treated with sh-NC or sh-circ102051. Tumor growth was suppressed when hsa_circRNA_102051 was suppressed. (C) H&E staining, KI67 staining and TUNEL staining were used to verify the proliferation and apoptosis of tumor cells in different treatment groups (x200). (D) The mRNA expressions of E-cadherin, N-cadherin and vimetin in different tumor tissues were detected by QRT-PCR. (E) Immunofluorescence was used to detect the expression of E-cadherin and vimetin in the two groups of tumor tissues (x200). (F) Number of intestinal tumors and tumorigenesis in colorectal cancer induction model. (G) H&E and fluorescence staining of E-cadherin and vimentin in mouse tissues (x200). Compared to sh-NC group, ^*P < 0.05