Fig. 5

BPTF was positively regulated by hsa_circRNA_102051 while negatively affected by miR-203a. (A) mRNA and protein expression of BPTF in SW480 and HT-29 cells with different levels of hsa_circRNA_102051. The higher hsa_circRNA_102051 level is, the more expression BPTF displayed. (B) mRNA expression of BPTF in tumor and corresponding adjacent tissue. (C) IHC staining of BPTF in tumor and corresponding adjacent tissue. BPTF was overexpressed in CRC samples. (D) mRNA and protein expression of BPTF in FHC cells and four CRC cell lines (SW480, HT-29, SW1463, HCT116). BPTF was overexpressed in CRC cell lines. (E) miRNAs interacting with both hsa_circRNA_102051 and BPTF were predicted by Starbase and Circular RNA Interactome. miR-203a was the mediate factor between hsa_circRNA_102051 and BPTF. (F) Relation analysis based on COAD data. BPTF expression was positively correlative to miR-203a level, R = -0.248. (G) MiR-203a expression in tumor and corresponding adjacent tissue. MiR-203a was downregulated in CRC. (H) Relation analysis within tissue samples. BPTF expression was positively correlative to miR-203a level, R = -0.799. (I) FISH staining of miR-203a and hsa_circRNA_102051. MiR-203a and hsa_circRNA_102051 was co-located in cytoplasm of colorectal cells. Compared to adjacent samples or normal cells, ^*P < 0.05