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Fig. 8 | Cancer Cell International

Fig. 8

From: Hsa_circRNA_102051 regulates colorectal cancer proliferation and metastasis by mediating Notch pathway

Fig. 8

Hsa_circRNA_102051/miR-203a/BPTF axis modulated stemness of CRC cells by affecting Notch pathway. (A) qRT-PCR of SOX9, OCT-4 and CD44 in CRC cells transfected with vector, hsa_circRNA_102051, miR-203a, or hsa_circRNA_102051 plus miR-203a. (B) Western blot of SOX9, OCT-4 and CD44 in four groups of CRC cells. Expression levels of SOX9, OCT-4 and CD44 were all enhanced by hsa_circRNA_102051 while inhibited by miR-203a, which were reversed in the hsa_circRNA_102051 plus miR-203a group. (C) Tumor sphere formation assays in four groups of CRC cells. Tumor formation was activated by hsa_circRNA_102051 while suppressed by miR-203a treatment. The hsa_circRNA_102051 plus miR-203a group had similar number of tumor spheres to the negative control. (D) Western blot of Notch 1, Hey 1 and Hes 1 in CRC cells with different transfections. Notch signals were activated by hsa_circRNA_102051 while blocked by miR-203a, and were both reversed in the hsa_circRNA_102051 plus miR-203a group. Compared to NC group, *P < 0.05

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Cancer Cell International

ISSN: 1475-2867

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