Fig. 8

PGRN activated the JAK/STAT signaling pathway. (A, B) Representative images and quantitation of the western blotting showed that the protein expression of JAK2, p-JAK2, STAT3, p-STAT3, STAT4 and p-STAT4 in the PGRN overexpression/knockdown groups (n = 3). GAPDH was used as an internal control. Data are presented as mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001. (C) Representative images and quantitation of the western blotting showed that the protein expression of JAK2, p-JAK2, p-STAT3, p-STAT4, BAD、BCL2、BAX、CyclinD1、E-Cadherin、N-Cadherin、vimentin and MMP2 in the PGRN overexpression group with JAK inhibitor (JSI-124) (n = 3). GAPDH was used as an internal control. Data are presented as mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001