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Fig. 4 | Cancer Cell International

Fig. 4

From: MALAT1/ mir-1-3p mediated BRF2 expression promotes HCC progression via inhibiting the LKB1/AMPK signaling pathway

Fig. 4

Knockdown of BRF2 inhibits HCC progression. (a, b) The transfection efficiency of si1/2-MALAT1 in Huh7 and Hep3B cells was detected by RT-qPCR and western blot. (c, d) After silencing BRF2, cell viability of Huh7 and Hep3B cells was detected by CCK-8 assay on days 0, 1, 2, 3, 4 and 5. (e) Colony formation assays were used to determine the role of BRF2 in HCC cell colony formation. (f-h) Transwell assay and cell scratch assay were used to detect migration and invasion of hepatocellular carcinoma cells after BRF2 down-regulation. (i) The apoptosis rate of HCC cells after BRF2 knockdown was detected by flow cytometry. (j) Western blot analysis was performed to detect the expression levels of apoptosis-related proteins in HCC cells after BRF2 knockdown. ** P < 0.01, *** P < 0.001

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Cancer Cell International

ISSN: 1475-2867

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