Fig. 2

Comparison of NUCB-2 expression in different NPC cell lines (CNE1, CNE2, 5-8 F and 6-10B), while Hela cell was used as the positive control and NP69 as the control of normal immortalized nasopharyngeal mucosal cell. (A) Presentation of NUCB-2 expression determined by Western blotting in different NPC cell lines and the controls; GAPDH was detected as an internal control of each sample. (B) Comparison of NUCB-2 expression in different NPC cell lines and the controls. The relative optical density of NUCB-2 expression level was obtained as a ratio of NUCB-2 to the internal control of GAPDH in western blot analysis. *: P < 0.05; ns: non-significant with P > 0.05