Fig. 3
From: NUCB-2/Nesfatin-1 promotes the proliferation of nasopharyngeal carcinoma cells
Suppressed NUCB-2 expression and its inhibition on CNE2 cell proliferation. (A) Western Blot detection on the effect of NUCB-2 expression in NUCB-2 knockdown clones of CNE2-KD-4, CNE2-KD-5 and CNE2-KD-6; GAPDH was employed as the internal control of each sample. (B) MTT assay. The x-axis represents the culture time of cells. The y-axis represents the absorbance of the cultured cells at the wavelength of 490 nm. (C) EdU assay. The figure displays the EdU fluorescence of CNE2-KD-5 cells. Blank: untreated CNE2 cells. (D) The results of the plate clone formation. (E) Histogram of the EdU results with the ImageJ software production, where the y-axis indicates the percentage of the labeled cells. (F) Histogram of the clone number of different CNE2 cell lines. (G) Histogram of the clone area (mm2). The ImageJ software was used for statistical analysis. Data are presented as the mean ± standard error. CNE2-NC: negative control, CNE2 cells transfected with the empty vector; *: P < 0.05; ns: no statistical difference