Fig. 5

Icariin-Curcumol attenuates DTX resistance by regulating the PI3K-Akt signaling pathway and Warburg effect. (A) Determination of cell viability at 0, 24, and 48 h by CCK8 assay. (B) Analysis of apoptosis by flow cytometry. (C) The relative levels of AR, PSA, PI3K, Akt1, mTOR, and HIF-1α were determined. (D) Determination of glucose, lactate and ATP content. (E) Determination of PDH and LDH levels. (F) The protein abundance of Glut1, Glut4, and MCT4 was examined. (G) The relative levels of c-Myc, hnRNPs, VEGF, HK2, PFK1, and PKM2 were detected. *P < 0.05 compared with the Model group; #P < 0.05 compared with the DTX group