Fig. 4

(A) Numerical data from the evaluation provided was used to visualize characteristics of dividing cells. To generate the plots, the track results table was sorted by start type and end type. Only cells with start and end type Split were displayed. Grouping was set to Object. Cell area or (B) mean intensity DPC were plotted against cell age. Tracked Cells-Age[s]: Time passed since the cell was first observed in seconds; Tracked Cells-Cell Area[µm²]: Average cell size over all cells per well; Intensity Cell Mean -Digital Phase Contrast: Intensity DPC signal, first averaged over all pixels per cell and then averaged over all cells per well. (C) Total length of all segments of an object track in µm; averaged over all tracks per well. (D) Distance from the first to last observation of a cell track in µm; averaged over all tracks per well. (E) Accumulated distance in µm divided by the track duration in seconds; calculated per track and then averaged over all tracks per well. *p < 0.05, ** p < 0.01 and *** p < 0.001