Fig. 5

(A) The white arrows indicate pseudopodia around the cells. Images of cytoskeletal staining were acquired with a confocal laser scanning microscope (1000x magnification, scale bar: 10 μm). (B) Scanning electron microscopy was used to observe the cell morphology. Compared with shNC group, the cell morphology and cell pseudopodia of shPTBP1 group were changed. Images were captured by scanning electron microscopy (1.00 K, 3.00Kx magnification, scale bar: 50 μm and 10 μm). (C) Image J software was used to analyze the mean fluorescence intensity of F-actin. (D) Transmission electron microscopy was used to observe GC cells. Compared with shNC group, microfilaments in shPTBP1 group changed from neatly arranged to disordered, indicating that actin skeleton remodeling was inhibited. On the left is a simple diagram of microfilament changes. The red arrows on the right indicate microfilaments in cells. Images were captured by transmission electron microscopy (1.50 K, 8.00Kx magnification, scale bar: 5 μm and 1 μm). (E) Western blot was used to detect the expression levels of PTBP1 and Paxillin in GC cells. (F) Immunofluorescence assays were conducted to detect the expression of PTBP1 and Paxillin in GC cells with PTBP1knockdown (200x magnification, scale bar: 50 μm). *p<0.05, ** p < 0.01 and ***p<0.001