Fig. 8

(A) Multiple immunofluorescence technology was used to stain GC tissue microarray, and automatic quantitative pathological imaging system was used to photograph, observe and quantitatively analyze (2.5, 10x magnification, scale bar: 200 μm and 50 μm). (B) The number of different cells was counted separately to obtain their distribution in the tumor, and then the differential expression of the whole tissue cancer and the paracancer was analyzed. (C) The differential expression of whole tissue carcinoma and paracancer was analyzed under the condition of positive PTBP1 and CK double staining. (D) The number of different cells were counted to obtain their distribution in the tumor, and then the differences in epithelium and stromal expression were analyzed. (E) Univariate overall survival was analyzed using Kaplan-Meier survival analysis and log-rank statistical test. * p < 0.05, *** p < 0.001