Fig. 3

DSCC1 promotes LUAD cell proliferation and stemness. (A-B) The data was collected from LinkedOmics. Gene: DSCC1; Sample cohort: TCGA_LUAD; Institute: UNC; Data type: RNA seq; Platform: HiSeq RNA; Statistical Method: Pearson correlation test; Patients: 515; Enrichment Analysis: GSEA; Rank Criteria (from LinkFinder Result): FDR. (C) LUAD cells were transfected with control siRNA or siDSCC1 for 48 h, and the expression of DSCC1 was determined. (D) LUAD cells were transfected with control siRNA or siDSCC1 for the indicated times, and SRB was used to detect cell proliferation. (E) The data was obtained from Sangerbox 3.0 (http://sangerbox.com/home.html). Cancer stemness and gene expression: Gene: DSCC1; Data source: RNAss; Data transformation: log2(x + 0.001); Pearson. (F,G) LUAD cells were transfected with DSCC1 siRNA and control siRNA and the colony formation was performed. (H) The data was obtained from UALCAN. (I) LUAD cells were transfected with DSCC1 siRNA and control siRNA for 48 h, and the mRNA level of indicated proteins were detected by quantitative PCR analysis. (J) LUAD cells were transfected with DSCC1 overexpressing plasmid and empty vector for 48 h, and the mRNA level of indicated proteins were detected by quantitative PCR analysis. (K) LUAD cells were transfected with DSCC1 siRNA and control siRNA for 48 h, and the expression of indicated proteins were detected by western blot