Fig. 5
From: Targeting MYH9 represses USP14-mediated NAP1L1 deubiquitination and cell proliferation in glioma
MYH9 inhibits NAP1L1 degradation by stabilizing USP14- NAP1L1 complex. RT-qPCR analysis and WB of NAP1L1 expression in U87 and LN229 cells transfected with siMYH9. (B) WB was used to detect the effects of DMSO or MG132 treatment and CHX treatment for different duration on the stability of NAP1L1 protein in the control and siMYH9 groups. (C) Co-IP detected the effects of MYH9 knockdown on protein stability of NAP1L1 in LN229 cells. (D) Co-IP detected the interaction of MYH9 and NAP1L1 with USP14. (E) Co-IP detected the effects of USP14 overexpression on protein stability of NAP1L1 in MYH9 overexpression in LN229 cells. Data are presented as the mean ± SD for three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001