Fig. 1
From: ARF6 promotes hepatocellular carcinoma proliferation through activating STAT3 signaling
ARF6 is highly expressed in HCC, and elevated ARF6 expression correlates with poor prognosis. (A, B) Immunohistochemical staining (IHC) and expression scoring of ARF6 was conducted in 169 HCC tissues. Representative pictures were shown (scale bar: 200 μm). (C, D) Kaplan-Meier analysis was applied to demonstrate the correlation between ARF6 expression and overall survival or disease-free survival of HCC patients. The cutoff for distinguishing low or high ARF6 expression was the median value. (E) The protein level of ARF6 was analyzed in additional 136 paired HCC tissues (tumor, T) with corresponding adjacent non-cancerous tissues (normal, N) by western blot. Representative western blot results were shown. (F) Statistical analysis showed that ARF6 was upregulated in HCC tissues, compared with that in normal liver, where GAPDH was used as a control. (G) Statistical analysis showed that ARF6 bands of HCC tissues were quantified and shown in the bar chart after being normalized to the respective adjacent non-tumor tissues