Fig. 2

Active ARF6 promotes cell proliferation, colony formation and upregulated cell cycle proteins in HCC cells. (A, B) CCK8 assay was performed in indicated cells. (C, D) Colony formation assay was performed in indicated cells. Representative images of colonies were shown (upper panel) and the number of colonies were counted (lower panel). (E, F) Western blot analysis of the level of cyclin D1, and p21 in indicated cells stably knockdown or overexpression of ARF6, compared with the control groups. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001: the scramble group compared with the shARF6#2 group