Fig. 4
From: ARF6 promotes hepatocellular carcinoma proliferation through activating STAT3 signaling
Active ARF6 promotes STAT3 signaling pathway activation. (A, B) Western blot analysis of the phosphorylation level of STAT3 in indicated HCC cells. (C) The phosphorylation level of STAT3 and ARF6 protein expression were analyzed in the same paired HCC tissues (tumor, T) with corresponding adjacent non-cancerous tissues (normal, N) by western blot. Representative western blot results were shown. (D) Statistical analysis showed that phosphorylated STAT3 was upregulated in HCC tissues, compared with that in normal liver, where GAPDH was used as a control. (E) Statistical analysis showed that phosphorylated STAT3 bands of HCC tissues were quantified and shown in the bar chart after being normalized to the respective adjacent non-tumor tissues. (F) Spearman correlation analysis between ARF6 and phosphorylated STAT3 level (N = 136)