Fig. 5
From: ARF6 promotes hepatocellular carcinoma proliferation through activating STAT3 signaling
STAT3 signaling is necessary for the oncogenic role of ARF6 in HCC in vitro. (A, B) Hep3B and Huh7 stably overexpressing ARF6Q67L cells were transiently transfected siRNA targeting STAT3 or treated with Stattic (2µmol/L). Cell viability of indicated cells were measured by CCK-8 assay. (C, D) Indicated cells were subjected to colony formation assay. Representative images of colonies were shown and the number of colonies were counted. (E, F) Western blot analysis of phosphorylated STAT3, STAT3, cyclin D1, P21 in indicated ARF6Q67L-overexpressed HCC cells transiently transfected siSTAT3 or treated with Stattic. *P < 0.05, **P < 0.01, ***P < 0.001