Fig. 6

STAT3 signaling is necessary for the oncogenic role of ARF6 in HCC in vivo. (A) Huh7 cells stably transfected with control or ARF6^Q67L were injected into the fank of nude mice. After the tumors grew to 3–5 mm in diameter, mice were treated with Stattic (50 mg/kg, three times per week for 4 weeks). Representative images of subcutaneous tumors from the two groups were shown. (B) Volume of subcutaneous tumors from the two groups were measured at indicated days after injection (n = 5). (C) Tumor volume and weight were compared between the two groups after the mice sacrificed. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. (D) The expression of ARF6, P21, Ki67 and p-STAT3 in indicated subcutaneous xenografts confirmed with HE staining was determined by IHC (scale bar: 200 μm). (E) expression scoring of above these factors was conducted. ns: no significance, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001