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Fig. 5 | Cancer Cell International

Fig. 5

From: The pyrazole derivative of usnic acid inhibits the proliferation of pancreatic cancer cells in vitro and in vivo

Fig. 5

Derivative 5 induces the death of pancreatic cancer cells. A-D: PANC-1 (A, C) or Mia PaCa-2 (B, D) cells were treated with DMSO (control, C), 5 (upper graphs—A, B) or UA (lower graphs—C, D) at 1 or 5 µg/mL for 48 h. The amounts of live (L), early apoptotic (EA), late apoptotic (LA), and necrotic (N) cells were determined by flow cytometry after staining with Muse™ Annexin V and Dead Cell Kit. The results are presented as the mean ± SE of 3–6 independent experiments. Statistical significance between the respective control and 5-treated fractions was determined with ANOVA and Sidak’s post hoc test and is marked with a (P < 0.0001), b (P < 0.001), and c (P < 0.05). E: PANC-1 and F: Mia PaCa-2 immunoblots for caspase-cleaved PARP. The blots were stripped and reprobed with an anti-β -actin antibody to ensure equal protein loading

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Cancer Cell International

ISSN: 1475-2867

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