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Table 2 Derivative 5 blocks pancreatic cancer cell cycle progression in G0/G1 phase in a time-dependent manner

From: The pyrazole derivative of usnic acid inhibits the proliferation of pancreatic cancer cells in vitro and in vivo

Name

Concentration [µg/mL]

Cell cycle phase [%]

G0/G1

S

G2/M

PANC-1

 Control

43.8 ± 1.3

20.4 ± 0.9

34.5 ± 1.7

 ( +) usnic acid

1

44.3 ± 2.6ns

20.5 ± 0.8ns

33.8 ± 3.4ns

5

42.7 ± 1.1ns

18.9 ± 1.2ns

36.3 ± 1.9ns

 5

1

47.8 ± 2.3ns

18.8 ± 2.1ns

31.9 ± 1.2 ns

5

61.9 ± 4.3a

8.1 ± 2.2b

28.4 ± 2.3ns

Mia PaCa-2

 Control

 

42.3 ± 1.1

29.9 ± 1.7

33.5 ± 1.2

 ( +) usnic acid

1

5

39.8 ± 1.4ns

41.2 ± 2.7ns

24.3 ± 3.6ns

24.9 ± 3.6 ns

33.2 ± 2.4ns

31.4 ± 2.1ns

 5

1

48.0 ± 1.5ns

19.2 ± 1.7ns

29.5 ± 2.6ns

5

54.5 ± 0.8a

15.7 ± 0.9ns

26.9 ± 1.7c

  1. Cells were treated with 1 or 5 µ g/mL UA or derivative 5 for 24 h. Each value is the mean (± SE) of three experiments. Statistical significance was determined with ANOVA and Sidak’s multiple comparison test and a – P < 0.0001, b – P < 0.001, c – P < 0.05, ns – not significant