Fig. 3

Pharmacological blockade of WEE1 by AZD1775 abrogates the G2-M cell cycle checkpoint and induces S-phase cell cycle arrest of ALL blasts. A Cell cycle analysis of a TP53 isogenic NALM6 cell line model treated for 24 h with DMSO or increasing doses of AZD1775. Histograms are representative of three independent experiments. 2N DNA content indicates cells in G₀ or G₁ phase. 4N DNA content indicates cells in either G₂ or M phase. Error bars show mean ± SD of at least three independent experiments. B Cell cycle analysis of high-risk TCF3::HLF-rearranged PDX#4 (TP53^wt), relapsed B-other PDX#19-r (TP53^wt), and relapsed hypodiploid PDX#10-r (TP53^mut) treated with DMSO or increasing doses of AZD1775. Hypodiploid cell DNA content (< 2N) was normalized to diploid DNA content (2N). PDX#4, 24 h; PDX#19-r and PDX#10-r, 48 h. Data are representative of one independent experiment for each sample