Fig. 3

USP5 drives EMT and metastasis in lung cancer. A Significant correlation between USP5 expression and the ssGSEA score of the SARRIO_EMT_UP gene set in the TCGA-LUAD dataset. r, Pearson correlation coefficient. B Representative images of CL1-5-shLacZ, shUSP5#1 and shUSP5#2 cells. Scale bar, 100 μm. C Protein levels of Slug, E-cadherin, N-cadherin, and Vimentin in CL1-5-shLacZ, shUSP5#1 and shUSP5#2 cells. β-actin was used as an internal control. D Wound healing analysis of CL1-5-shLacZ, shUSP5#1, and shUSP5#2 cells at 10 h. Scale bar: 200 μm. E Wound healing analysis of A549 cells stably expressing either vector or USP5 at 16 h. Scale bar: 200 μm. F Transwell invasion analysis of CL1-5-shLacZ, shUSP5#1, and shUSP5#2 cells. Scale bar: 200 μm. G Transwell invasion analysis of A549 cells stably expressing either vector or USP5. Scale bar: 200 μm. H In vivo bioluminescence images of NOD/SCID mice subcutaneously injected with Luc-CL1-5 cells expressing either shLacZ or shUSP5#2 at 21 days after injection (right). Photon counts per area were measured. In all plots, *P < 0.05 and **P < 0.01 by a two-tailed Student’s t test