Fig. 6

Kaplan-Meier survival curves of lung cancer patients stratified by the combined markers USP5 expression, Wnt signaling, and Stemness scores. Global gene expression data were obtained for 500 lung cancer patients in the TCGA-LUAD dataset. The ssGSEA scores for Wnt signaling and stemness were calculated with the R/Bioconductor package GSVA (v1.34.0) using the gene sets of REACTOME_TCF_DEPENDENT_SIGNALING_IN_ RESPONSE_TO_WNT and BENPORATH_ES_CORE_NINE, respectively. A The patients were divided into high and low groups for each factor using the median value as the cutoff. The combined effects of USP5 expression, Wnt signaling, and stemness on the overall survival of lung cancer patients were analyzed. The median survival of each molecular subtype is indicated. ***P < 0.001, **P < 0.01, *P < 0.05 by the log-rank test. B Relative proportions of patients categorized based on USP5 expression and Wnt signaling and Stemness scores