Fig. 5
RP11-367G18.1 variant 2–YY1 complex mediates hypoxia-induced EMT. (A and B) Knockdown of RP11-367G18.1 variant 2 or YY1 suppressed EMT, cell migration, and invasion of FADU and MCF7 cells overexpressing HIF-1α (ΔODD). (C and D) Knockdown of RP11-367G18.1 variant 2 or YY1 suppressed EMT, cell migration, and invasion of FADU and MCF7 cells under hypoxia. Glut1 and LDHA, the hypoxia-inducible genes, served as positive control. For hypoxic conditions, cells were cultured in 1% O2, 5% CO2, and 94% N2 for 18 h. Scr, Scrambled; Cont, control; V2, variant 2; N, normoxia; H, hypoxia. Data are represented as the mean ± SD. *P < 0.05