Fig. 7
RP11-367G18.1 variant 2–YY1 complex regulates gene expression. (A) The transcript expression levels of RP11-367G18.1 variant 2, YY1, HK2, TGFBI, VEGFC, and LIF were measured at the indicated time points (in hours) under hypoxic conditions in FADU cells. (B) The proteins expressions levels of HIF-1α, YY1 and H4K16Ac were measured at the indicated time points (in hours) under hypoxic conditions in FADU cells. (C) Knockdown of RP11-367G18.1 variant 2 or YY1 inhibited the expression of HK2, TGFBI. VEGFC, and LIF under hypoxia in FADU cells. (D) Knockdown of RP11-367G18.1 variant 2 suppressed the level of H4K16Ac and the binding of YY1 to the proximal promoters of HK2, TGFBI, VEGFC, and LIF under hypoxic conditions. Glut1, a hypoxia-inducible gene, served as positive control. For hypoxic conditions, cells were cultured in 1% O2, 5% CO2, and 94% N2 for 18 h. Cont, control; V2, variant 2. Data are represented as the mean ± SD. Student’s t-test, *P < 0.05