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Fig. 4 | Cancer Cell International

Fig. 4

From: Bladder cancer: therapeutic challenges and role of 3D cell culture systems in the screening of novel cancer therapeutics

Fig. 4

Summary of the main steps used for the preparation of three-dimensional (3D) culture models of cancer cells. (A) Dissociated cells are collected from primary tumors or cancer cell lines. (B) Obtained cancer cells are then incubated with an appropriate extracellular matrix, e.g., collagen, to ensure their 3D orientation. (C) A 3D organoid is formed. The diagram was created with BioRender [45]

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