Fig. 1

Isolation and identification of exosomes from clinical CRC serum samples. A The morphology of exosomes from clinical serum specimens identified by transmission electron microscopy (TEM). Scale bar: 200 nm; magnification: 30000 × ; red arrow: pointing to exosomes. B The diameter distribution of exosomes determined by nanoparticle tracking analysis (NTA). C The proportion of exosomes marked with positive TSG101/CD63 determined by flow cytometry. D The protein expression of TSG101 and CD63 (exosome surface markers) measured by western blotting (WB). CRC colorectal cancer, BID benign intestinal disease, HC healthy control. * (BID vs CRC), P < 0.05; ## (HC vs CRC), P < 0.01