Fig. 4

Effect of miR-653 knockdown and overexpression on CRC cell proliferation, migration, invasion, and apoptosis. A qR-PCR showing the relative expression level of miR-653 in CRC cells (* vs NCM460, P < 0.05; ** vs NCM460, P < 0.01). B qRT-PCR data showing the relative expression level of miR-653 in SW620 and HCT116 cells transfected with miR-NC and miR-653 mimic. C BrdU assay data showing the overexpression of miR-653 to reduce the percentage of BrdU positive cells. Red, SW620 and HCT116 cells labeled with BrdU; blue, nuclei counterstained by BrdU. Scale bar: 10 μm. D Colony formation assay showing the overexpression of miR-653 to weaken the clongenic ability. E, F Transwell assay showing the overexpression of miR-653 to suppress cell migration and invasion abilities. Scale bar: 8 μm. G Flow cytometry showing the overexpression of miR-653 to increase the percentage of apoptotic cells (** vs miR-NC, P < 0.01. NC negative control)