Fig. 6

Exosomal hsa-circ-0004771 in CRC cell culture medium mediates resistance to 5-fluorouracil by modulating miR-653/ZEB2 axis. qRT-PCR data showed: A1 Exosomal hsa-circ-0004771 expression was up-regulated in cell culture media from 5-FU-resistant CRC cells as compared to 5-FU-sensitive CRC cells. A2 The relative mRNA expression of miR-653 was down-regulated in 5-FU-resistant CRC cells as compared to 5-FU-sensitive CRC cells. A3 The relative mRNA expression of ZEB2 was up-regulated in 5-FU-resistant CRC cells as compared to 5-FU-sensitive CRC cells (P < 0.05). B1 Overexpression of miR-653 in 5-FU-resistant CRC cells suppressed the exosomal expression of hsa-circ-0004771 in cell culture media. B2 miR-653 was overexpressed in 5-FU-resistant CRC cells transfected with miR-653 mimic. B3 Overexpression of miR-653 in 5-FU-resistant CRC cells down-regulated ZEB2 expression in cells (P < 0.05). C The relative mRNA expression of ZEB2 in 5-FU-resistant CRC cells transfected with siNC/si-hsa-circ-0004771/si-hsa-circ-0004771 + miR-653 mimic. (* vs siNC, P < 0.05; # vs si-hsa-circ-0004771, P < 0.05). mRNA messenger RNA, ZEB2 zinc finger E-box binding homeobox 2, NC negative control, si small interfering