Fig. 4

FAP suppresses apoptosis and enhances ccRCC progression. A FAP content in the ccRCC cell lines (Caki-1, ACHN, A498) and normal cell line (HK-2). B QRT-PCR was used for quantifying FAP expression levels in Caki-1 cells and ACHN cells upon transfection. C, D Flow cytometry was used to measure Caki-1 as well as ACHN cell apoptotic activities post-transfection. E, F After transfection, the CCK-8 assays were performed to detect cellular viability in Caki-1 and ACHN cell lines at 24, 48, 72, and 96 h. * p < 0.05 vs. the Ctrl group