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Fig. 6 | Cancer Cell International

Fig. 6

From: Long intergenic non-coding RNA DIO3OS promotes osteosarcoma metastasis via activation of the TGF-β signaling pathway: a potential diagnostic and immunotherapeutic target for osteosarcoma

Fig. 6

Knockdown of DIO3OS inhibited cellular migration, invasion of osteosarcoma cells in vitro. A Expression level of DIO3OS in osteosarcoma cell lines and healthy cell line detected by qRT-PCR. B The location of DIO3OS in SaoS-2 cell line was determined by qRT-PCR of nuclear and cytoplasmic RNA fraction isolations. C DIO3OS expression was inhibited in SaoS-2 cells after si-DIO3OS transfection. D DIO3OS expression was inhibited in U2OS cells after si-DIO3OS transfection. E Cell migration and invasion was inhibited in SaoS-2 cells transfected with si-DIO3OS. The scale bar were marked in red, 100 μm. F Cell migration and invasion was inhibited in U2OS cells transfected with si-DIO3OS. The scale bar were marked in red, 100 μm. G The expression levels of E-cad protein were increasd and Vimentin protein expression were inhibited in SaoS-2 cells after si-DIO3OS transfection. H The expression levels of E-cad were up-regulated and Vimentin protein were inhibited in U2OS cells after si-DIO3OS transfection. I DIO3OS knockdown suppressed cell migration capacity in SaoS-2 cells, as determined by wound healing assay. J DIO3OS knockdown suppressed cell migration capacity in U2OS cells, as determined by wound-healing assay. P-value of less than 0.05 is considered statistically significant (ns, p > 0.05; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001)

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Cancer Cell International

ISSN: 1475-2867

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