Fig. 2

MND1 promotes the proliferation of GC cells and induces cell cycle arrest in the G1 phase. A, B QRT-PCR and WB were used to detect MND1 expression in GC cells. C, D WB was used to detect the levels of MND1 after silencing and overexpression in GC cells. E The localization and silencing efficiency of MND1 cells were detected by immunofluorescence assay. F, G Colony forming and CCK-8 assays were used to detect the proliferation after silencing and overexpression of MND1. H EDU was used to detect cell proliferation after silencing and overexpression of MND1. I The changes of cell cycle related protein expression in each group were detected by WB. J, K Cell cycle changes were detected by flow cytometry after silencing and overexpression of MND1. (*P-value < 0.05, **P-value < 0.01, ***P-value < 0.001). All figures represent mean ± SD from three independent experiments