Fig. 3

MND1 promotes invasion, migration, and apoptotic escape of GC cells. A, B Cell migration and invasion abilities were determined by wound healing and transwell assays after silencing and overexpression of MND1 in GC cells. C WB was used to evaluate the effects of silencing and overexpression of MND1 on EMT-related proteins. D Annexin V-APC/PI double staining was used to detect apoptosis after silencing and overexpression of MND1. E WB was used to evaluate the effects of silencing and overexpression of MND1 on apoptosis. F Subcutaneous tumorigenesis in nude mice was performed to evaluate the effect of MND1 silencing on tumor proliferation. G Tumor growth curve and tumor weight in nude mice. H Immunohistochemistry was used to detect the expressions of Ki67, MND1, E-cadherin, N-Carherin and Vimentin in nude mice (*P-value < 0.05, **P-value < 0.01, ***P-value < 0.001). All figures represent mean ± SD from three independent experiments