Fig. 5

FOXA1 binds to the MND1 promoter and affects the progression of GC cells. A. Venn diagrams based on the hTFtarget and PROMO databases predicted the transcription factors bound to the MND1 promoter. B, C QRT-PCR and WB were used to detect the expressions of FOXA1 and MND1 after overexpression of FOXA1. D The double luciferase reporter gene assay showed the direct binding of FOXA1 to MND1. E, F CCK-8 and clone colony test were used to detect the proliferation in each group. G, H Cell migration and invasion abilities in each group was detected by wound healing and transwell assays. I Flow cytometry was used to detect the changes of cell cycle in each group. J Annexin V-APC/PI double staining was used to detect apoptosis in each group (*P-value < 0.05, **P-value < 0.01, ***P-value < 0.001). All figures represent mean ± SD from three independent experiments