Fig. 6

MND1 directly binds to TKT and regulates proliferation of GC cells. A The intersection genes were detected by Venn diagram based on mass spectrometry and flg-MND1 mass spectrum. B Peak plot of TKT in mass spectrometry. C, D QRT-PCR and WB were used to detect the expression of TKT after MND1 silencing. E MND1 and TKT localizations were examined by immunofluorescence. F CO-IP was used to detect the combination of TKT and MND1. G, H CCK-8 and cell cloning assay were used to detect the proliferation after MND1 silencing. I EDU was used to detect the proliferation after MND1 silencing. J Flow cytometry was used to detect the changes of cell cycle in each group. K Cell cycle-related proteins were detected by WB (*P-value < 0.05, **P-value < 0.01, ***P-value < 0.001). All figures represent mean ± SD from three independent experiments. 83 84 85