Fig. 7

TKT silencing inhibits metastasis and reduces lactic acid production and glucose uptake in GC cells. A, B Annexin V-APC/PI double staining and WB were used to detect apoptosis. C, D Wound healing assay and transwell assay were used to detect cell migration and invasion abilities. E EMT related proteins were detected by WB. F Lactic acid production was detected by the L-lactic acid detection kit. G The glucose uptake assay was performed by the glucose detection kit. H The TKT enrichment signaling pathway was detected by GSEA and the expressions of AKT, P-Akt, PI3K, P-PI3K and P53 were detected by WB (*P-value < 0.05, **P-value < 0.01, ***P-value < 0.001). All figures represent mean ± SD from three independent experiments